Optogenetics
All protocols/resources are provided for free use. While we make every attempt to ensure their accuracy and function, we make no representations or warranties of any kind, express or implied, about the completeness, accuracy, reliability, or suitability with respect to the information or products for any purpose. Any reliance you place on such information is therefore strictly at your own risk.
​
If you find these programs useful, we only ask you cite relevant publications from our laboratory
Protocol/Assembly
Our laboratory uses standard optogenetic constructs primarily obtained from the University of North Carolina Vector Core. In the process of combining optogenetic inhibition/excitation with behavior, a number of problems presented themselves, requiring custom solutions. By far the biggest problem was destruction of the optogenetic cable (Thor, Multimode FC/PC Fiber Optic Patch Cables with Ferrule Ends). Specifically, the ferrule end of this cable was very weak, and would break shortly into a behavior session.
To overcome this problem, we construct optogenetic cables with flexible metal shielding as follows:
​
We also have a protocol to make a bilateral, optogenetic cable:
BilateralCableAssemblyDiagram.pdf
​
In addition, we make, polish and calibrate all ferrule implants:
​
Use of these protocols has greatly lengthened the life of our optogenetic cables and has permitted cables to remain firmly plubgged in for entire behavioral sessions, upwards of 90 minutes. However, even after implementing this cable design, we would occasionally have rats come unplugged during a behavior session. We discovered this was largely due to the relatively small opening atop the Med Associates chamber. To overcome this problem we modified the tops of each chamber by enlarging the hole (to ~4 inches in diameter) and epoxying a funnel on top. The funnel tapers down to ~1.5 inches and is centered exactly below the ceiling-mounted commutator. This design permits the cable to move freely with little or no contact with the behavioral chamber.
​​
​
